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Gastrointestinal parasitism in livestock is still a relevant problem in some regions of the world. Serological tests that are based on the detection of circulating levels of circulating parasite antigen in the blood, known as antigen-ELISA and serum antibody-ELISA, are currently the most widely used techniques in the diagnosis of this disease. However, in many situations, these methods have shown low sensitivity, especially for small ruminants. Recently developed methods, such as western blot, are more sensitive but are much more time consuming and therefore often are not used in the routine diagnosis of the disease. Therefore, there is an urgent need for the development of improved, accurate and efficient diagnostic methods to detect small ruminant gastrointestinal parasites, which should be affordable and easy to perform. This review summarizes some of the diagnostic methods currently used for the diagnosis of gastrointestinal parasitism, including antigen-ELISA, serum antibody-ELISA, western blot and, from a practical point of view, the fecal egg count technique. Although these methods have been used for a long time, the results obtained are often unsatisfactory for the accurate and rapid diagnosis of parasitism. In this context, this review discusses some of the limitations of current methods and the obstacles that remain to overcome before they can be used reliably for parasite detection, especially in small ruminants.Glial-neuronal interactions in neuronal control of estrous cyclicity in rats.
Neurons of the sexually dimorphic medial preoptic nucleus (MnPO) are activated during sexual behavior and during the cycle-dependent changes of vaginal estrous mucus secretion. The specific involvement of neurons in the MnPO and of peptidergic and neurotrophic substances in their functional activity was investigated in the present study. Enkephalin immunoreactivity was evenly distributed throughout the lateral preoptic area and along the MnPO; synthetic neurotrophins (beta-nerve growth factor (beta-NGF) and ciliary neurotrophic factor (CNTF)) immunoreactivity was observed in the lateral preoptic area but not along the MnPO. Glial-type immunoreactivity to a monoclonal
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